Effects of Temperature on Enzyme Biology Introduction In order to understand the activity of enzymes at different temperatures the ability of the enzyme to function can be measured. This is important in many applications such as Polymerase Chain Reaction for forensics as well as genetics research where manipulation of temperature-dependent enzymes allows for replication of DNA segments. Bennett states, “when the energy – measured as heat – increases in a chemical reaction the energy increases the reaction speed and reaction rate. (1969) This happens until a threshold though in many biological processes where positive energy input no longer increases reaction speed due to the inhibition of other factors – in this experiment that factor is the degradation or denaturing of the protein complex that is the enzyme working to catalyze the reaction.

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